Purpose: Cycloporine A (CsA) is a immunosuppressive agent most widely used in organ transplanted patients for preventing immunorejection, but it has some side effects, including hypertension, nephrotoxicity, hepatotoxicity and diabetes.
The
mechanism of toxicity of CsA was not completely understood, but the reactive oxygen species has been proposed to be involved in the reaction of toxicity of CsA. The purpose of this study is to determine the effects of glutathione, as a
physiological
antioxidant on CsA induced cytotoxicity in rat insulinoma (RINm5F) cells.
Methods: RINm5F cells were incubated in the presence of CsA (105∼108 M) and buthionine sulfoximine (BSO), as a inhibitor of r-glutamyl cysteine synthetase, was
added
to
cultured media (RPMI1640). Twenty four hours of incubation with CsA and BSO, viable cells were determined by MTT method.
Results: CsA decreased cell viability in dose response in cultured RINm5F cells and significantly decreased according
to
redusing glutathione.
Conclusion: These results suggested that CsA may induce the diabetes and glutathione have some roles in the pathogenesis of CsA-induced diabetes.
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